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目的:观察强肌健力口服液与龟板混合血清对大鼠骨髓间充质干细胞(MSCs)成肌分化的影响。方法:使用密度梯度离心法分离大鼠MSCs,5-溴脱氧嘧啶(Brdu)和CD44双重免疫组化鉴定,传3代后的MSCs分为强肌健力口服液与龟板混合血清组和空白血清对照组,免疫组化染色鉴定肌凝蛋白(myosin)、结蛋白(desmin)的表达。结果:诱导后第3、13天,强肌健力口服液与龟板混合血清组部分细胞出现desmin和myosin阳性表达。结论:强肌健力口服液与龟板混合血清能诱导MSCs向成肌细胞定向分化,为中医脾肾相关理论提供依据。
OBJECTIVE: To observe the effects of Qiangji Jianli oral liquid and turtle-plate mixed serum on myogenic differentiation of rat bone marrow mesenchymal stem cells (MSCs). METHODS: The rat MSCs were isolated by density gradient centrifugation and identified by double immunohistochemistry using Brdu and CD44. Three passages of MSCs were divided into strong muscle Jianli oral liquid and turtle shell mixed serum group and blank serum. In the control group, the expression of myosin and desmin was identified by immunohistochemical staining. RESULTS: On the 3rd and 13th days after induction, desmin and myosin were positively expressed in some cells of Qiangji Jianli oral liquid and turtle plate mixed serum group. Conclusion: Qiangji Jianli Oral Liquid and turtle-plate mixed serum can induce directional differentiation of MSCs into myoblasts, which provides a basis for related theories of spleen and kidney in Chinese medicine.