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目的探讨补充维生素D、胶原肽和钙对大鼠骨骼发育的影响。方法健康雄性初断乳SD大鼠按体质量随机分为5组,其中1组为低钙对照组,实验期间给予低钙饲料喂养,其余4组在低钙饲料的基础上,分别给予含不同剂量的维生素D、胶原肽和钙的饲料喂养,12周后测定大鼠骨骼生长发育相关指标、血清骨代谢生化指标、钙表观吸收率,并对骨组织切片进行病理学观察。结果各受试物剂量组大鼠股骨中点骨密度为(1 889±0.011 0)g/cm2、(2 188±0.020 4)g/cm2、(1 908±0.011 4)g/cm2、(1 919±0.009 7)g/cm2,均显著高于低钙对照组,最大载荷分别为(96.95±11.85)、(122.81±13.67)、(97.48±8.94)、(100.45±19.51)N,显著高于低钙对照组,维生素D和碳酸钙联合干预组各指标优于其他各组;碳酸钙、维生素D和胶原肽三者联合干预组血清I型前胶原氨基端前肽(PINP)水平显著低于对照组;各组钙表观吸收率分别为(93.87±1.70)%、(93.72±1.20)%、(92.47±1.33)%、(90.45±2.81)%,其中2、3组比对照组(90.86±4.19)%高(P<0.05)。结论单独补充碳酸钙,或碳酸钙与维生素D和/或胶原肽联合补充均能够促进成长期雄性大鼠骨的发育,但碳酸钙、维生素D和胶原肽三者联合的干预效果未达预期,其机制有待进一步深入探讨。
Objective To investigate the effects of vitamin D supplementation, collagen peptide and calcium on bone development in rats. Methods Healthy male weanling SD rats were randomly divided into 5 groups according to their body weight. Among them, 1 group was low calcium control group, while low calcium diet was used during the experiment. On the other hand, Dose of vitamin D, collagen peptide and calcium feed, after 12 weeks of rat bone growth and development related indicators, serum biochemical markers of bone metabolism, apparent absorption rate of calcium, and bone histological sections were observed. Results The BMD of femur in each dose group was (1 889 ± 0.011 0) g / cm2, (2 188 ± 0.020 4) g / cm2, (1 908 ± 0.011 4) g / cm2, 919 ± 0.009 7) g / cm2, were significantly higher than those in the low calcium control group, with the maximum loads of (96.95 ± 11.85), (122.81 ± 13.67), (97.48 ± 8.94) and (100.45 ± 19.51) N, The indexes of low calcium control group, vitamin D and calcium carbonate combined intervention group were better than those of other groups. The levels of serum type I procollagen amino terminal propeptide (PINP) were significantly lower than those of the combination of calcium carbonate, vitamin D and collagen peptide (93.82 ± 1.20)%, (92.47 ± 1.33)% and (90.45 ± 2.81)% respectively in the control group, and the apparent absorption rates of calcium in the two groups were (93.87 ± 1.70)% and ± 4.19)% (P <0.05). CONCLUSION: Calcium carbonate alone or calcium carbonate combined with vitamin D and / or collagen peptide could promote the development of bone in adult male rats. However, the combination of calcium carbonate, vitamin D and collagen peptide did not reach the expected effect. Its mechanism needs to be further explored.