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目的观察藤莓汤对胶原诱导性关节炎(collagen-induced arthritis,CIA)大鼠滑膜过氧化物酶体增殖物激活受体-γ(peroxisome proliferator activated receptor gamma,PPARγ)、核转录因子-κB(nuclear factor kappa B,NF-κB)、IL-17表达的影响,探讨该方抑制类风湿关节炎(rheumatoid arthritis,RA)滑膜免疫炎性病理损伤的分子机制。方法建立SD大鼠CIA模型,将成模大鼠随机分为模型组、西药组、中药高剂量组、中药低剂量组,并设正常组,每组6只。正常组与模型组予去离子水10 m L/(kg·d)灌胃,西药组予来氟米特1.87mg/(kg·d)灌胃,中药高、低剂量组分别以藤莓汤生药量31.8、15.9 g/(kg·d)灌胃,连续干预12周。干预结束后检测PPARγ、P65、IL-17蛋白及mRNA表达。结果与正常组比较,模型组PPARγ、P65、IL-17 mRNA及蛋白表达上调(P<0.01)。与模型组比较,中药高剂量组PPARγ蛋白表达上调,P65、IL-17 mRNA及蛋白表达下调,差异有统计学意义(P<0.01);西药组及中药低剂量组PPARγmRNA及蛋白表达上调,P65、IL-17 mRNA及蛋白表达下调,差异有统计学意义(P<0.01)。结论藤莓汤可改善CIA大鼠关节滑膜病理损伤,抑制免疫炎性因子表达。
Objective To observe the effects of Fujuipin decoction on the expression of peroxisome proliferator activated receptor gamma (PPARγ), nuclear factor kappa B (NF-κB) in collagen-induced arthritis (CIA) (NF-κB) and IL-17 in synovial tissue of rheumatoid arthritis (RA) to investigate the molecular mechanism of the inhibition of immunoinflammatory pathological changes in rheumatoid arthritis (RA) synovium. Methods CIA model was established in SD rats. The rats were randomly divided into model group, western medicine group, high-dose Chinese medicine group and low-dose Chinese medicine group. Normal rats were divided into six groups. Normal group and model group were treated with deionized water (10 m L / (kg · d)) orally, leflunomide 1.87 mg / (kg · d) Raw drugs 31.8,15.9 g / (kg · d) intragastric administration, continuous intervention for 12 weeks. After intervention, the expression of PPARγ, P65, IL-17 protein and mRNA were detected. Results Compared with normal group, the mRNA and protein expressions of PPARγ, P65 and IL-17 in model group were up-regulated (P <0.01). Compared with model group, the expression of PPARγ protein in high-dose group was up-regulated and the expressions of P65, IL-17 mRNA and protein were down-regulated (P <0.01); the expression of PPARγ mRNA and protein were up-regulated in western medicine group and low- , IL-17 mRNA and protein were down-regulated (P <0.01). Conclusion Fujifilm can improve the pathological damage of synovium and inhibit the expression of immunoinflammatory cytokines in CIA rats.