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【目的】明确引起广西蔗区甘蔗花叶病的病原,为甘蔗抗花叶病育种、种质筛选及病害防控提供科学依据。【方法】从广西主要蔗区采集不同品种显症或不显症甘蔗叶片,提取RNA后采用甘蔗花叶病毒(SCMV)、高粱花叶病毒(Sr MV)、玉米矮化病毒(MDMV)和约翰逊草花叶病毒(JGMV)特异引物进行一步法RT-PCR检测,将目的片段克隆并测序;病毒CP基因序列在Gen Bank数据库中进行比对分析。【结果】在收集的48份样品中检测出Sr MV阳性33份,SCMV阳性2份,SCMV和Sr MV复合侵染1份,未检测到MDMV和JGMV。花叶病的发病率为75.0%,其中,Sr MV、SCMV及两种病毒复合侵染的发病率分别为68.8%、4.2%和2.1%。台糖系列及其他引进品种的甘蔗花叶病感病率较高,桂糖系列品种的感病率相对较低。【结论】甘蔗花叶病已在广西大部分蔗区发生,其致病原以Sr MV为主,SCMV零星发生,由SCMV和Sr MV复合侵染的甘蔗花叶病发生机率较低。广西蔗区可能存在其他种类病原病毒引起的甘蔗花叶病。
【Objective】 The objective of this study was to identify the pathogen of sugarcane mosaic disease in sugarcane sugarcane in Guangxi and to provide a scientific basis for the breeding of anti-mosaic disease, germplasm screening and disease prevention and control of sugarcane. 【Method】 The sugarcane leaves of different varieties of indica or insignificant species were collected from the main sugarcane growing areas of Guangxi. RNA was extracted from sugarcane mosaic virus (SCMV), sorghum mosaic virus (MV), maize dwarf virus (MDMV) and Johnson The specific primers of grass mosaic virus (JGMV) were detected by one-step RT-PCR. The target fragment was cloned and sequenced. The CP gene sequence of the virus was compared in Gen Bank database. 【Result】 Among the 48 samples collected, 33 positive samples of Sr MV, 2 positive samples of SCMV and 1 composite of SCMV and Sr MV were detected. MDMV and JGMV were not detected. The incidence of Mosaic disease was 75.0%. Incidence rates of Sr MV, SCMV and two viruses were 68.8%, 4.2% and 2.1% respectively. Taiwan sugar series and other varieties introduced higher susceptibility to sugarcane mosaic disease, Gui sugar series is relatively low susceptibility. 【Conclusion】 Sugarcane mosaic disease occurred in most of sugarcane growing areas in Guangxi. The pathogen was mainly Sr MV. SCMV sporadically occurred. The incidence of sugarcane mosaic disease was low in combination with SCMV and Sr MV. Sugarcane mosaic disease caused by other kinds of pathogenic viruses may exist in Guangxi sugarcane field.