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Objective To explore the immune stimulation effect of recombinant E.coli LLO/OVA on mice bone marrow-derived dendritic cells (BMDCs) and T lymphocytes in vitro.Methods After BMDCs stimulated by E.coli LLO/OVA,their Toll-like receptor (TLR) and nucleotide-binding oligomerization domain (NOD) receptor signalling pathway were examined by superarray hybridization;and the priming effect of the vaccine activated BMDCs on CD4+T and CD8+T was determined by [3H]thymidine uptake and ELISA,the tumor cytotoxic effect of activated CD8+T cells was determined by cytotoxic assay.Results After BMDCs were activated by E.coli LLO/OVA via TLR4,NOD1 receptor and NF-κB signalling pathway,the expression of their surface molecules including MHC class Ⅰ,MHC class Ⅱ,CD40,CD80 and CD86 significantly up-regulated;the secretion of IL-12 and IFN-? increased also.The mature BMDCs stimulated the allergic CD4+T and CD8+T cells proliferation and their IL-2 and IFN-γ secretion,and the activated CD8+T cells effectively killed B16-OVA melanoma cells and RMA-S/OVA lymphoma cells in vitro.Conclusion E.coli LLO/OVA is effective in inducing BMDCs maturation via activating TLR4 and NOD1 receptor signalling pathway and promoting specific anti-tumor T cell immunity in vitro.
Objective To explore the immune stimulation effect of recombinant E. coli LLO / OVA on mice bone marrow-derived dendritic cells (BMDCs) and T lymphocytes in vitro. Methods After BMDCs stimulated by E. coli LLO / OVA, their Toll-like receptor ( TLR) and nucleotide-binding oligomerization domain (NOD) receptor signaling pathway were examined by superarray hybridization; and the priming effect of the vaccine activated BMDCs on CD4 + T and CD8 + T was determined by [3H] thymidine uptake and ELISA, the tumor cytotoxic effect of activated CD8 + T cells was determined by cytotoxic assay. Results After BMDCs were activated by E. coli LLO / OVA via TLR4, NOD1 receptor and NF-κB signaling pathway, the expression of their surface molecules including MHC class I, MHC The secretion of IL-12 and IFN- increased also. The mature BMDCs stimulated the allergic CD4 + T and CD8 + T cells proliferation and their IL-2 and IFN-γ secretion, and the activated CD8 + T cells effecti vely killed B16-OVA melanoma cells and RMA-S / OVA lymphoma cells in vitro. Conclusion E. coli LLO / OVA is effective in inducing BMDCs maturation via activating TLR4 and NOD1 receptor signaling pathway and promoting specific anti-tumor T cell immunity in vitro .