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目的 探讨cag致病岛在中国人幽门螺杆菌 (Hp)感染者中的分布特征及其与Hp致病的相关性。方法 采用PCR方法检测了 10 7例临床分离培养的Hp菌株 ,分别扩增cagA中的 2 98bp片段、cag致病岛的cagⅠ中的 170bp及cagⅡ中的 5 97bp片段。结果 cag致病岛的阳性检出率为95 3% ,其中cagⅠ和cagⅡ均阳性的检出率为 6 1 7% ,单纯cagⅠ或cagⅡ阳性的检出率分别为 2 5 2 %和 7 5 % ,并发现 1株仅有cagA存在、cagⅠ其他部分和cagⅡ均未检出的Hp。 5例cag致病岛阴性的Hp菌株中 ,4例来源于慢性胃炎 ,1例来源于贲门癌。cag致病岛的检出在慢性胃炎、消化性溃疡、胃癌等疾病间差异无显著性 (P >0 0 5 )。结论 cag致病岛在中国人群感染Hp者中的检出率很高 ,且以cagⅠ和cagⅡ共存为主 ,少部分表现为cagⅠ或cagⅡ的缺失。cag致病岛的存在或部分缺失与其致病性的相关性并不显著 ,但cag致病岛阴性的Hp主要集中于慢性胃炎患者 ,提示cag致病岛仍具有一定的致病意义
Objective To investigate the distribution of cag pathogenicity island in Chinese patients with Helicobacter pylori (Hp) infection and its correlation with Hp pathogenesis. Methods A total of 107 strains of Hp strains isolated from clinical isolates were detected by polymerase chain reaction (PCR). Two hundred and seventy-eight bp fragments of cagA, 170 bp in cagⅠ and 579 bp in cagⅡ were amplified respectively. Results The positive rate of cag pathogenicity island was 95 3%, of which the positive rate of cagⅠ and cagⅡ was 61.7%. The positive rates of cagⅠ or cagⅡ were only 25.5% and 75% respectively , And found that only one cagA exists, cag I and cag Ⅱ were not detected in other parts of the Hp. Of the 5 Hp isolates with cag pathogenicity, 4 were from chronic gastritis and 1 were from cardia. The detection of cag pathogenicity island in chronic gastritis, peptic ulcer, gastric cancer and other diseases showed no significant difference (P> 0.05). Conclusions The detection rate of cag pathogenicity island is high in Chinese population infected with Hp, and mainly cagⅠ and cagⅡ coexist. A small number of cagⅠ or cagⅡ are missing. The existence or partial deletion of cag pathogenicity island was not significantly related to its pathogenicity, but the Hp negative for cag pathogenicity island mainly focused on patients with chronic gastritis, suggesting that cag pathogenicity island still has some pathogenic significance