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探讨人乳腺球蛋白(human mammary globulin,hMAM)在乳腺癌前哨淋巴结(sentinel lymph node,SLN)中的表达及其临床意义。RT-PCR检测20例乳腺癌组织及正常淋巴结组织中hMAM的表达;SLN定位后通过RT-PCR与常规病理检测SLN转移情况,并进行比较;分析SLN转移与临床病理的关系。乳腺癌组织中有18例过表达,表达率为90.0%,而正常淋巴结中hMAM不表达,两者差异有统计学意义(P<0.01)。常规病理检查转移率为50.98%,RT-PCR法检测的阳性率提高到70.59%,RT-PCR法比常规病理检测的检出率高19.61%,两者之间差异显著(χ2=38.28,P<0.01)。SLN无转移组、微转移组、转移组与肿瘤位置、ER表达及病理类型之间差异均不显著(P>0.05)。hMAM可作为辅助判断SLN是否转移的标志。
To investigate the expression of human mammary globulin (hMAM) in sentinel lymph node (SLN) and its clinical significance. The expression of hMAM was detected by RT-PCR in 20 cases of breast cancer and normal lymph node tissues. The SLN metastasis was detected by RT-PCR and routine pathology after SLN mapping. The relationship between SLN metastasis and clinical pathology was analyzed. Eighteen cases of breast cancer were overexpressed, the expression rate was 90.0%, while the expression of hMAM in normal lymph nodes was not statistically different (P <0.01). The rate of routine pathological examination was 50.98%, and the positive rate of RT-PCR was 70.59%. The detection rate of RT-PCR was 19.61% higher than that of routine pathological examination (χ2 = 38.28, P <0.01). There was no significant difference in SLN metastasis, micrometastasis, metastasis, tumor location, ER expression and pathological type (P> 0.05). hMAM can be used as a secondary marker to determine whether SLN is metastatic or not.