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目的研究湖北土家族人群HLA-DRB1基因多态性,了解其分布特征,为开展群体遗传学相关研究奠定基础。方法采用聚合酶链反应-序列特异性寡核苷酸探针(PCR-SSOP)流式细胞技术对342份无血缘关系健康的湖北恩施土家族人群样本进行HLA-DRB1等位基因分型检测,分析等位基因分布频率,并与其他地区人群进行比较。结果共检出13种等位基因,其中以DRB1*09(0.174 0)、DRB1*04(0.136 0)、DRB1*12(0.125 7)、DRB1*15(0.118 4)、DRB1*08(0.109 6)最为常见,未检出DRB1*03(18)。湖北土家族人群HLA-DRB1等位基因总体分布分别与湖南苗族人群比较P>0.05;分别与昆明汉族、云南纳西族比较P<0.05;分别与湖北汉族、内蒙古鄂温克族、宁夏回族、内蒙古蒙古族、西藏珞巴族、昆明彝族、新疆克尔克孜族比较,P<0.01。结论了解湖北恩施土家族人群的HLA-DRB1的遗传多态性,对于追溯土家族起源和民族间的血缘遗传关系具有重要意义。
Objective To study the polymorphism of HLA-DRB1 gene in Tujia nationality in Hubei Province, and to understand the distribution characteristics of HLA-DRB1 gene, so as to lay the foundation for the study of population genetics. Methods The HLA-DRB1 alleles were genotyped by polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP) in 342 samples of Enshi Tujia ethnic group with no blood relationship. The frequency of allele distribution was analyzed and compared with those in other regions. RESULTS: Thirteen alleles were detected in all the patients. DRB1 * 09 (0.174 0), DRB1 * 04 (0.136 0), DRB1 * 12 (0.125 7), DRB1 * 15 ) Was the most common and DRB1 * 03 (18) was not detected. The overall distribution of HLA-DRB1 alleles in Tujia nationality in Hubei Province was respectively compared with those in Miao nationality in Hunan Province (P> 0.05), respectively, compared with Han nationality in Kunming and Naxi ethnic groups in Yunnan (P <0.05). The distributions of HLA-DRB1 alleles were similar to those of Hubei Han, Inner Mongolia, , Tibet Lhoba, Kunming Yi and Xinjiang Krks, P <0.01. Conclusion It is important to understand the genetic polymorphism of HLA-DRB1 in Enshi Tujia ethnic groups in Hubei Province, which is of great significance for tracing the origin of Tujia ethnic group and the genetic relationship among ethnic groups.