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目的了解Ｃ基因启动子区Ｔ１７６２Ａ１７６４变异对其启动子的影响。方法ＰＣＲ产物直接测序，检测重症乙型肝炎患者乙型肝炎病毒（ＨＢＶ）前Ｃ和Ｃ基因调节序列的Ｔ１７６２Ａ１７６４变异；并将该调节序列插入到氯霉素乙酰转移酶（ＣＡＴ）表达质粒中，转染ＨｅｐＧ２细胞并瞬时表达，比较Ｔ１７６２Ａ１７６４野株及变异株的Ｃ基因启动小区序列及ＣＡＴ表达水平。结果Ｔ１７６２Ａ１７６４变异在使ＣＡＴ表达下调中起主要作用。结论Ｔ１７６２Ａ１７６４变异使Ｃ基因启动子活性下调中起重要作用。
Objective To investigate the effect of mutation of T-1762A1764 in promoter region of C gene on its promoter. Methods The PCR products were directly sequenced to detect the T1762A1764 variation in the regulatory sequences of pre-C and C genes of hepatitis B virus (HBV) in patients with severe hepatitis B. The regulatory sequence was inserted into the expression plasmid of chloramphenicol acetyltransferase (CAT) HepG2 cells were transfected and transiently transfected. The promoter region and CAT expression level of C gene in T1762A1764 wild-type and mutant strains were compared. Results The variation of T1762A1764 played a major role in down-regulation of CAT expression. Conclusion The mutation of T1762A1764 plays an important role in the down-regulation of promoter activity of C gene.