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利用水、旱稻杂交、回交所产生的4个分离群体对RIL群体定位到的抗旱相关的根基粗、抗旱系数2个QTL进行了选择验证。结果表明,根基粗、抗旱系数QTL的两侧标记在不同群体、不同的遗传背景中遗传稳定。在旱田种植条件下,YIBC1J、IBC1和JIF23个分离群体携带有根基粗QTLbrt4.1两侧标记有利等位基因的个体与未携带brt4.1两侧标记有利等位基因的个体均值差为0.05~0.06 mm,均达到显著或极显著水平,MAS效应为5.05%~6.12%;利用抗旱系数QTLidr6.1两侧标记进行选择时,入选个体的单株产量均值差为5.04~8.18 g,均达到显著或极显著水平,MAS效应为34.89%~58.88%。用根基粗、抗旱系数2个QTL的两侧标记同时进行选择时,入选个体的根基粗、单株产量均值差分别为0.06~0.08 mm、4.88~11.15 g,同样达到显著或极显著水平,MAS效应分别高达6.06%~8.16%和39.91%~70.48%。旱田3个分离群体基于双标记、单标记选择结果表明,发生在brt4.1、idr6.1标记区间的单交换值分别为4.32%、10.27%,利用brt4.1、idr6.1单侧标记进行选择也获得了较好的选果。在水田种植条件下,YIBC1群体携带有根基粗、抗旱系数QTL两侧标记有利等位基因的个体与没有携带QTL有利等位基因的个体均值差异均不显著,表明brt4.1和idr6.1为旱田特异表达。4个分离群体的选择结果验证了QTL定位的真实性、可靠性。另外,还对QTL及其两侧标记的选择效应及水旱稻抗旱育种策略进行了探讨。
Four QTLs derived from drought and waterlogging hybridization and backcross were used to validate the two QTLs for drought resistance related to the RIL populations. The results showed that both sides of QTL with coarse roots and drought-resistance coefficients were stable in different populations and genetic backgrounds. Under upland cultivation conditions, the mean difference between individuals carrying YIBC1J, IBC1 and JIF23 individuals with the favorable alleles flanking both sides of the crude QTLbrt4.1 and the favorable alleles not carrying the two sides of brt4.1 was 0.05 ~ 0.06 mm, respectively. The MAS effect was 5.05% -6.12%. When selecting the markers with QTLidr6.1 drought resistance coefficient, the mean difference of yield per plant was 5.04-8.18 g, all of which were significant Or extremely significant level, MAS effect was 34.89% ~ 58.88%. When both sides of two QTLs with rough root and drought resistance coefficient were selected for selection at the same time, the average root yield of the selected individuals were 0.06-0.08 mm and 4.88-11.15 g, respectively, reaching significant or extremely significant levels. MAS The effects were as high as 6.06% ~ 8.16% and 39.91% ~ 70.48% respectively. The results showed that the single exchange values of brt4.1 and idr6.1 were 4.32% and 10.27%, respectively. The brt4.1 and idr6.1 unilateral markers Choice also received better results. Under paddy field cultivation, there was no significant difference in the mean of individuals carrying YQLT1 alleles between YIBC1 population with individuals with or without QTL, indicating that both brt4.1 and idr6.1 were Dry field-specific expression. The results of the selection of four segregating populations verified the authenticity and reliability of QTL mapping. In addition, the selection effect of QTLs and markers on both sides and drought and drought breeding strategies of paddy and upriver were also discussed.