目的:研究建立咽舒喷雾剂质量控制的方法。方法:用薄层色谱法鉴别山豆根、黄芩、牡丹皮;用高效液相色谱法对牛蒡子苷进行定量分析。结果:定性鉴别分离度好,专属性强;牛蒡子苷在0.13~32.50μg范围内线性关系良好(r=0.999 9,n=5),回收率98.66%(RSD为1.19%,n=9)。结论:建立的定性、定量方法快速、准确、专属性强,可作为咽舒喷雾剂的质量控制方法。 Objective: To study the method of establishing the quality control of Yanshu Spray. Methods: Identification of Soybean Root, Scutellaria baicalensis and Cortex Moutan by TLC. Quantitative analysis of Arctiin by HPLC. Results: The results showed that the identification of arctiin was good and the specificity was good. The arctiin had a good linearity (r = 0.999 9, n = 5) in the range of 0.13 ~ 32.50 μg and the recovery was 98.66% (RSD 1.19%, n = 9) . Conclusion: The established qualitative and quantitative methods are rapid, accurate and specific, and can be used as quality control method for pharyngeal swab.