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目的对姜黄素进行了相应的修饰得到了二十五个姜黄素衍生物,并在细胞水平对这些化合物的体外抗肿瘤活性及其机制进行初步探讨。方法采用MTT法研究姜黄衍生物(6.25μM、12.5μM、25μM和50μM)在体外对HL-60的成活率的影响,并采用吖啶橙(AO)/溴化乙锭(EB)荧光双染法染色及流式细胞术对其作用机制进行了初步探讨。结果MTT实验结果表明姜黄素衍生物对人白血病细胞株HL-60的存活率具有一定的影响,其中9#、12#作用最强,且具有时间和剂量依赖性。AO/EB荧光双染法染色及流式细胞术(PI)结果表明姜黄素衍生物9#、12#可以显著的诱导HL-60细胞凋亡,并呈剂量和时间依赖性。结论姜黄素衍生物9#、12#可能通过诱导细胞凋亡抑制细胞生长。
Twenty-five curcumin derivatives were obtained by the corresponding modification of curcumin, and the in vitro antitumor activities of these compounds at cellular level were also discussed. Methods MTT assay was used to study the effects of turmeric derivatives (6.25μM, 12.5μM, 25μM and 50μM) on the survival rate of HL-60 in vitro. The results were analyzed by acridine orange (AO) / ethidium bromide The mechanism of action staining and flow cytometry were discussed. Results MTT results showed that curcumin derivatives have a certain impact on the survival rate of human leukemia cell line HL-60. Among them, 9 # and 12 # have the strongest effect and are time-and dose-dependent. AO / EB double staining staining and flow cytometry (PI) results show that curcumin derivatives 9 #, 12 # can significantly induce HL-60 cell apoptosis in a dose and time-dependent manner. Conclusion Curcumin derivatives 9 #, 12 # may inhibit cell growth by inducing apoptosis.