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[目的]观察氟中毒大鼠骨组织中Notch3、Jag1蛋白表达水平,探讨其在氟骨症发生机制中的作用。[方法]SD大鼠24只随机分为对照组、低氟组、高氟组3组,每组8只。对照组自由饮用自来水,低氟组、高氟组分别饮用含50、100 mg/L氟化钠的自来水。饲养6个月后处死大鼠。用氟离子选择电极法分别测定尿氟和骨氟,观察骨组织病理学改变,免疫组化检测成骨细胞Notch3、Jag1蛋白表达,免疫印迹法检测骨组织Notch3、Jag1蛋白表达,采用实时荧光定量PCR法检测骨组织Notch3、Jag1 mRNA表达水平。[结果]与对照组相比较,低氟组、高氟组大鼠尿氟、骨氟水平明显增高(均P<0.05)。光镜观察染氟组大鼠出现骨硬化。高氟组大鼠骨组织Notch3蛋白水平与mRNA相对表达含量明显低于对照组(均P<0.05);高氟组大鼠骨组织Jag1蛋白水平和mRNA相对表达含量明显低于对照组、低氟组(均P<0.05)。免疫组化法检测显示低氟组和高氟组大鼠成骨细胞Notch3、Jag1蛋白水平均明显低于对照组(均P<0.05)。[结论]过量氟抑制骨组织特别是成骨细胞Notch3、Jag1表达,使成骨作用增强,该过程可能参与氟骨症发病机制。
[Objective] To observe the expression of Notch3 and Jag1 protein in the bone tissue of fluorosis rats and to explore its role in the pathogenesis of skeletal fluorosis. [Method] Twenty-four SD rats were randomly divided into control group, low fluoride group and high fluoride group, with 8 rats in each group. Control group drinking tap water freely, low fluoride group, high fluoride group drinking 50,100 mg / L sodium fluoride respectively. Rats were sacrificed 6 months after feeding. Urinary fluoride and bone fluoride were measured by fluoride ion selective electrode method. The pathological changes of bone were observed. The expression of Notch3 and Jag1 protein in osteoblasts was detected by immunohistochemistry. The expressions of Notch3 and Jag1 protein in bone were detected by immunoblotting. PCR method was used to detect the mRNA expression of Notch3 and Jag1 in bone tissue. [Results] Compared with the control group, urinary fluoride and bone fluoride levels were significantly increased in low fluoride group and high fluoride group (all P <0.05). Light microscope observation of fluorine-exposed rats with osteosclerosis. Compared with control group, the content of Notch3 protein and mRNA in bone tissue of high fluoride group was significantly lower than that of control group (all P <0.05); the content of Jag1 protein and mRNA in bone tissue of high fluoride group was significantly lower than that of control group, Group (all P <0.05). Immunohistochemistry showed that the protein levels of Notch3 and Jag1 in osteoblasts of low fluoride group and high fluoride group were significantly lower than those in control group (all P <0.05). [Conclusion] Excessive fluoride inhibits the expression of Notch3 and Jag1 in osteoblasts and osteoblasts, which may be involved in the pathogenesis of skeletal fluorosis.