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AIM:Bd-2/adenovirus E1B 19 ku interacting protein 2-like(BNIPL-2) is a novel protein recently identified in ourlaboratory.BNIPL-2 is homologous to human BNIP-2,apotentially proapoptotic protein,and can interact with Bcl-2 and Cdc42GAP and promote apoptosis in BEL-7402 cells.Here we report the gene-expression profile regulated byBNIPL-2 in human hepatocarcinoma Hep3B cells and theanalysis of its potential roles in cell apoptosis.METHODS:BNIPL-2 was overexpressed in Hep3B cellsusing tetracycline inducible or Tet-on system.Screened byWestern blot,the cells with low background and highinduction fold of BNIPL-2 were obtained.We performedAtlas human cDNA expression array hybridization on thesecells and analyzed the data with Quantarray~(?) software toidentify BNIPL-2-regulated genes and their expressionprofile.RT-PCR was used to confirm the altered expressionlevel of part of genes identified by the Atlas array hybridization.RESULTS:Fifteen of 588 genes spotted on the Atlasmembrane showed altered expression levels in BNIPL-2-transfected Hep3B-Tet-on cells,in which 8 genes involvedin cell apoptosis or growth inhibition were up-regulatedand 7 genes involved in cellular proliferation were down-regulated following overexpression of BNIPL-2.CONCLUSION:cDNA array is a powerful tool to exploregene expression profiles under inducible conditions.Thedata obtained using the cDNA expression microarraytechnology indicates that BNIPL-2 may play its roles inapoptosis through regulating the expression of genesassociated with cell apoptosis,growth inhibition and cellproliferation.
AIM: Bd-2 / adenovirus E1B 19 ku interacting protein 2-like (BNIPL-2) is a novel protein recently identified in our laboratory. BNIPL-2 is homologous to human BNIP-2, apotentially proapoptotic protein, and can interact with Bcl- 2 and Cdc42GAP and promote apoptosis in BEL-7402 cells. Here we report the gene-expression profile by BNIPL-2 in human hepatocarcinoma Hep3B cells and the analysis of its potential roles in cell apoptosis. METHODS: BNIPL-2 was overexpressed in Hep3B cells using tetracycline inducible or Tet-on system. Screened by Western blot, the cells with low background and high induction fold of BNIPL-2 were obtained. We performed Atlas human cDNA expression array hybridization on these cells and analyzed the data with Quantarray® software to identify the BNIPL-2 -regulated genes and their expression profile. RT-PCR was used to confirm the altered expression level of part of the objects identified by the Atlas array hybridization .RESULTS: Fifteen of 588 genes spotted on the Atlasmembrane showed alt ered expression levels in BNIPL-2-transfected Hep3B-Tet-on cells, which 8 genes involved in cell apoptosis or growth inhibition were up-regulated and 7 genes involved in cellular proliferation regulated-down overexpression of BNIPL-2.CONCLUSION: cDNA array is a powerful tool to exploregene expression profiles under inducible conditions. Tdata obtained using the cDNA expression microarraytechnology indicates that BNIPL-2 may play its roles inapoptosis through regulating the expression of genesassociated with cell apoptosis, growth inhibition and cellproliferation.