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为研究FaGAMYB基因在草莓成花诱导和花发育中的作用,以‘卡姆罗莎’草莓(Fragaria×ananassa Duch.‘Camarosa’)为试材,采用同源克隆和RT-PCR技术分离了一个赤霉素信号途径中的MYB转录因子基因FaGAMYB。该基因开放阅读框为1 689bp,编码562个氨基酸。序列分析发现FaGAMYB蛋白含有高度保守的R2R3DNA结合域,以及GAMYB基因家族所特有的Box1,Box2和Box3保守区域。亚细胞定位研究发现FaGAMYB蛋白定位于洋葱表皮细胞的细胞核。FaGAMYB基因具有组成型表达特性,但在雄蕊、花芽、茎尖生长点和果实等生殖生长组织与器官的表达丰度较高。在草莓成花诱导期,茎尖中FaGAMYB基因表达上调,当进入雄蕊原基分化期后,该基因表达量迅速升高。上述结果表明FaGAMYB可能在草莓成花诱导和雄蕊发育中发挥重要作用。
In order to study the role of FaGAMYB gene in floral induction and flower development in strawberry, Fragaria × ananassa Duch.’Camarosa ’was used as a test material, and homologous cloning and RT-PCR techniques were used to isolate one The MYB transcription factor gene FaGAMYB in gibberellin signaling pathway. The open reading frame of this gene is 1 689 bp, encoding 562 amino acids. Sequence analysis revealed that the FaGAMYB protein contains highly conserved R2R3 DNA binding domains as well as Box1, Box2 and Box3 conserved regions specific to the GAMYB gene family. Subcellular localization study found FaGAMYB protein located in the nucleus of onion epidermal cells. The FaGAMYB gene has constitutive expression characteristics, but its expression abundance is higher in the reproductive growth tissues and organs such as stamens, flower buds, shoot tips and fruits. During strawberry induction, the expression of FaGAMYB gene in shoot tip was up-regulated. The expression of FaGAMYB gene was rapidly increased after entering the stamen primordium differentiation stage. The above results indicate that FaGAMYB may play an important role in flowering induction and stamen development in strawberry.