在常规实验室检测产肠毒素大肠杆菌(ETEC)需要简易、快速的检测方法。目前,最常用的方法是用DNA杂交和多聚酶链反应(PCR)技术通过检测编码肠毒素产物的基因而确定。然而,采用多聚核苷酸或寡核苷酸探针进行菌落印迹杂交所获结果不均一;而PCR检测粪便样本时,则存在受到粪便杂质抑制的不足。本文对寡核苷酸探针进行粪便样本的DNA杂交和PCR技术作对比试验和评价。 Detection of enterotoxigenic Escherichia coli (ETEC) in routine laboratories requires a simple and rapid assay. Currently, the most commonly used method is to determine by DNA hybridization and polymerase chain reaction (PCR) techniques the gene encoding enterotoxin production. However, colony blot hybridization using polynucleotides or oligonucleotide probes resulted in nonuniformity, whereas PCR detection of stool samples impaired the control of stool impurities. In this paper, oligonucleotide probe stool samples DNA hybridization and PCR techniques for comparative testing and evaluation.