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目的探讨补肾益肺消癥方对肺纤维化(IPF)大鼠外周血中T淋巴细胞亚群分化的影响及作用机制。方法通过博莱霉素致IPF模型,观察补肾益肺消癥方对IPF大鼠症状和体征的改变,通过流式细胞术检测CD3+、CD3+CD4+、CD3+CD8+T淋巴细胞以及CD4+/CD8+T淋巴细胞比值的变化。结果补肾益肺消癥方可以明显改善IPF大鼠的症状和体征,治疗组与模型组对比体重明显增加,差异有极显著性(P<0.01)。阳性对照组T淋巴细胞总数显著下降,出现明显的免疫抑制作用;中药各剂量组可以明显地降低IPF大鼠的外周血中CD8+T淋巴细胞的含量,小剂量和大剂量组与模型组相比有极显著性差异(P<0.01);中剂量和大剂量组CD4+/CD8+T淋巴细胞比值相比模型组和空白组显著升高,差异有极显著性(P<0.01)。结论补肾益肺消癥方通过干预IPF患者T淋巴细胞亚群的分化,调控病变肺组织的免疫应答反应,进而延缓肺组织纤维化损伤的进程,达到治疗效果。
Objective To investigate the effect of Bushen Yifei Xiaozhang Recipe on T lymphocyte subpopulation differentiation in peripheral blood of rats with pulmonary fibrosis (IPF) and its mechanism. Methods The IPF model induced by bleomycin was used to observe the symptoms and signs of Bushen Yifei Xiaozheng Recipe on IPF rats. The expressions of CD3 +, CD3 + CD4 +, CD3 + CD8 + T lymphocytes and CD4 + / CD8 were detected by flow cytometry + T lymphocyte ratio changes. Results Bushen Yifei Xiaozheng Decoction could obviously improve the symptoms and signs of IPF rats. The body weight of rats in treatment group and model group increased obviously, the difference was significant (P <0.01). The total number of T lymphocytes in the positive control group decreased significantly, showing obvious immunosuppressive effect; each dose of traditional Chinese medicine can significantly reduce the content of CD8 + T lymphocytes in the peripheral blood of IPF rats, (P <0.01). The ratios of CD4 + / CD8 + T lymphocytes in medium dose group and high dose group were significantly higher than those in model group and blank group (P <0.01). Conclusion Bushen Yifei Xiaozheng Recipe can regulate the immune response of lung tissue by intervening the differentiation of T lymphocyte subsets in patients with IPF, and then delay the process of lung fibrosis and achieve the therapeutic effect.