论文部分内容阅读
目的对婴幼儿腹泻A组轮状病毒的三种不同检测方法进行评价。方法同时采用胶体金、琼脂糖凝胶电泳、逆转录PCR(RT-PCR)对浙江萧山医院1周320份腹泻患儿的标本进行轮状病毒检测,然后进行方法学比较。结果三种方法的阳性检出率分别为36.3%、48.1%和42.8%,经卡方检验三种方法的检出率之间差异均存在统计学意义(P<0.05);以琼脂糖凝胶电泳为检测的最终标准,RT-PCR的敏感性为88.9%,特异性达100%;胶体金法的敏感性71.4%,特异性达96.4%。结论胶体金法检测轮状病毒的敏感性和特异性能够满足临床上对轮状病毒快速筛检的要求;PT-PCR法的敏感性和特异性均最高,既可用于临床检测,又是轮状病毒血清G、基因P分型的重要手段,在对无菌体液和环境样本中轮状病毒的检测具有不可比拟的优势。
Objective To evaluate three different detection methods of group A rotavirus in infants with diarrhea. Methods Colloidal gold, agarose gel electrophoresis and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect rotavirus in 320 children with diarrhea in one week in Zhejiang Xiaoshan Hospital, and then compared by methodology. Results The positive detection rates of the three methods were 36.3%, 48.1% and 42.8%, respectively. There was significant difference between the three methods (P <0.05) Electrophoresis was the final standard of detection. The sensitivity and specificity of RT-PCR were 88.9% and 100% respectively. The sensitivity and sensitivity of colloidal gold method were 71.4% and 96.4% respectively. Conclusion The sensitivity and specificity of colloidal gold assay for detection of rotavirus can meet the clinical requirements for rapid screening of rotavirus. The sensitivity and specificity of PT-PCR are the highest, which can be used for clinical testing and round The important means of typing serogroup G and genotyping P has an unparalleled advantage in the detection of rotavirus in sterile body fluids and environmental samples.