论文部分内容阅读
目的探讨金胺O荧光染色后用LED显微镜镜检结核分枝杆菌的假阳性率和原因。方法选取宁乡县疾病预防控制中心2015年金胺O荧光染色LED镜检初涂阳性的痰标本用酸性罗氏培养基简单法进行分离培养。结果全年132例金胺O荧光染色镜检初涂阳性标本经培养证实共有3例假阳性病例,假阳性率为2.3%。AFB阳性(报菌数)13例,AFB阳性(+)16例,AFB阳性(++)35例,AFB阳性(+++)46例,AFB阳性(++++)22例。结论 LED镜检假阳性集中出现在镜检报告菌量较少的标本中,食物残渣等其他抗酸物质和标本交叉污染是产生假阳性的主要原因。
Objective To investigate the false positive rate and cause of Mycobacterium tuberculosis after fluorescent microscopy with Auramide O microscopy. Methods Ningxiang County Center for Disease Control and Prevention in 2015 Auramine O fluorescent staining LED smear-positive sputum specimens with acidic Roche media simple method for isolation and culture. Results A total of 132 false positive cases were found by fluorescence microscopy in 132 cases of Au amine O fluorescence staining. The false positive rate was 2.3%. AFB was positive in 13 cases, AFB was positive in 16 cases, AFB was positive in 35 cases, AFB was positive in 46 cases, AFB was positive in 22 cases. Conclusion The false positive results of LED microscopy appear in specimens with less microscopic examination reports. The main cause of false positives is the cross contamination of other acid-resistant substances such as food debris and specimens.