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自Aschheim及Zondek(1928)首先建立生物学妊娠试验以来,检测绒毛膜促性腺激素(human chorionic gonadotropin,hCG)用以诊断和随访某些疾病已成为妇产科临床实践中的主要方法。近60年来随着免疫学的不断发展,有关hCG的检测方法发展很快。迄今为止hCG的检测方法大体可分为:(1)生物学测定,(2)免疫试验;(3)放射免疫测定 (Radioimmunoassay,RIA);(4)放射受体测定(Radioreceptorassay,RRA);(5)酶免疫吸附分析;(6)荧光免疫分析法等。本文主要综述近20年来hCG测定方法的进展及其临床应用。一、检测hCG方法的进展 (一)放射免疫测定 RIA法是60年代初发展起来的一种放射性同位素技术与免疫化学技术相结合的体外测定超微量物质的新技术。RIA法应用hCG检测是Wide及Gemzdl(1960)首创。目前用于放射免疫
Since Aschheim and Zondek (1928) first established a biological pregnancy test, the detection of human chorionic gonadotropin (hCG) for the diagnosis and follow-up of certain diseases has become a major method in the clinical practice of obstetrics and gynecology. With the continuous development of immunology in the past 60 years, the detection method of hCG has developed rapidly. So far, detection methods of hCG can be divided into: (1) biological assay, (2) immunoassay, (3) radioimmunoassay (RIA), (4) Radioreceptorassay (RRA) 5) enzyme immunoassay; (6) fluorescence immunoassay and so on. This article mainly summarizes the progress of hCG determination in the past 20 years and its clinical application. First, the detection of hCG method of progress (a) radioimmunoassay RIA method is developed in the early 60’s a radioisotope technology combined with immunochemical techniques in vitro determination of trace substances in new technology. The RIA method using hCG detection is Wide and Gemzdl (1960) first. Currently used for radioimmunoassay