【摘 要】
：
Background and Aims: Numerous studies have explored the important role of N6-methyladenosine (m6A) in cancer. Nonetheless, the interaction between m6A and long noncod-ing RNAs (lncRNAs) is poorly investigated. Herein, we system-atically analyzed the role
【机 构】
：
Department of Hepatic Surgery and Liver Transplant Program,The Third Affiliated Hospital of Sun Yat-
论文部分内容阅读
Background and Aims: Numerous studies have explored the important role of N6-methyladenosine (m6A) in cancer. Nonetheless, the interaction between m6A and long noncod-ing RNAs (lncRNAs) is poorly investigated. Herein, we system-atically analyzed the role and prognostic value of m6A-related lncRNAs in hepatocellular carcinoma (HCC). Methods: The m6A-related lncRNAs were identified based on the correlation coefficients with m6A-related genes in HCC from The Cancer Genome Atlas. Subsequently, a novel risk score model was determined using the least absolute shrinkage and selection operator Cox regression analyses. Univariate and multivari-ate Cox analyses were used to identify independent prog-nostic factors for overall survival (OS) of HCC; thereafter, a prognostic nomogram was constructed. Results: A total of 259 lncRNAs showed significant correlations with m6A in HCC, while 29 lncRNAs had prognostic significance. Further, six critical m6A-related lncRNAs (NRAV, SNHG3, KDM4A-AS1, AC074117.1, AC025176.1, and AL031985.3) were screened out to construct a novel risk score model which classified HCC patients into high- and low-risk groups. Survival analy-ses revealed that patients in the high-risk group exhibited worse OS, both in the training and validation groups. The risk score was also identified as an independent prognostic factor of OS, and a nomogram was established and verified with superior prediction capacity. Besides, the risk score signifi-cantly correlated with the expression of immune checkpoint genes and immune subtypes. Conclusions: These findings indicated the significant role of m6A-related lncRNAs in HCC and the potential application of the novel risk score model for prognostic prediction.
其他文献
[背景]针对循环系统疾病,此前研究多集中在空气污染、气象因素对它的影响,忽略了城市建成环境会影响循环系统疾病的发生.[目的]探讨中国城市建成环境对循环系统疾病死亡的影响,并量化其影响效果.[方法]以中国17个城市为研究对象,收集2000—2019年循环系统疾病死亡数据和城市建成环境数据(涉及城市绿化、城市用地、城市土地混合、城市道路设施、城市医疗设施).采用多元线性回归方法分析循环系统疾病死亡率与建成环境的关系,并进行定量分析.深入探究当城市经济发展水平相异、空气质量状况不同的情况下,建成环境会对循环系统
[背景]腐霉利(PCM)暴露可致雄性小鼠生殖器官损伤,但其是否与视黄酸(RA)信号通路有关尚不明确.[目的]探讨RA信号通路在PCM致青春期小鼠睾丸损伤中的表达特征.[方法]取64只健康雄性ICR小鼠(3周龄)适应性饲养1周后,随机均分为对照和低、中、高剂量染毒组,每组16只,连续21 d经口灌胃,给予PCM剂量分别为0、50、100、200 mg·kg-1.停止给药1周后处死小鼠,取其血清和双侧睾丸,检测血清中睾酮含量,观察睾丸切片中的组织学变化,用实时荧光定量PCR法、Western blotting
全氟化合物(PFASs)是一类存在于环境和生物体中的持久性有机污染物.PFASs由于具有优良的疏水疏油性和化学稳定性,被广泛应用于各种工业和消费产品.PFASs可以通过食物链不断蓄积并产生生物放大作用,对人类健康产生不良影响,其中免疫毒性可能是PFASs暴露最敏感的健康效应之一,已引起广泛关注.本文归纳了PFASs免疫毒性的国内外流行病学研究,包括PFASs对免疫系统的抑制和过度激活作用;综述了PFASs对免疫器官、免疫细胞和免疫因子等方面影响的实验研究;并进一步总结了PFASs免疫毒性可能的作用机制,包
Nonalcoholic fatty liver disease (NAFLD) is a multisystemic clinical condition that presents with a wide spectrum of ex-trahepatic manifestations, such as obesity, type 2 diabe-tes mellitus, metabolic syndrome, cardiovascular diseases, chronic kidney dise
Defects in mitochondria are responsible for various ge-netic and acquired diseases. Mitochondrial transplantation, a method that involves introduction of healthy donor mi-tochondria into cells with dysfunctional mitochondria, could offer a novel approach
Background and Aims: Intrahepatic cholangiocarcinoma (ICC) is the second most common primary hepatic malig-nancy that causes a poor survival. We aimed to identify its prognostic factors and to develop a nomogram that will pre-dict survival of ICC patients
目的 探讨卵圆孔未闭(patent foramen ovale,PFO)合并偏头痛患者行经股静脉介入封堵术的临床疗效和安全性.方法 回顾性研究乐山市人民医院心脏外科2018年5月至2019年2月收治的PFO合并偏头痛并行经股静脉介入封堵术患者163例,比较所有患者术前、术后1、6个月经胸心脏超声心动图右心声学造影结果;比较所有患者术前、术后1、3、6个月头痛发作频率、持续时间、头痛程度评分.统计所有患者术后6个月手术疗效.结果(1)所有患者均获随访,随访时间为6～12个月,平均(7.24±3.72)个月;
[背景]大气二氧化氮(NO2)暴露可能会增加小于胎龄儿(SGA)和大于胎龄儿(LGA)的发生风险.然而,既往研究在推测孕期大气污染暴露影响的易感窗口时,主要是将孕期划分为相对较长的暴露时期,如孕早、中、晚期或者妊娠月.目前尚无研究探索过怀孕前后大气NO2周均暴露水平与SGA和LGA发生风险的关联.[目的]评估孕前和孕期大气NO2暴露对SGA和LGA发生风险的影响,并细化暴露窗口为每周,以更为准确地分析NO2暴露的易感窗口.[方法]本研究依托在天津市开展的“环境和生活因素在人整个生命轨迹中对代谢健康的影响研
Background and Aims: With high rates of recurrence post-treatment, hepatocellular carcinoma (HCC) is one of the most common types of cancer worldwide and the major cause of cancer death. To improve the overall sur-vival of HCC patients, identification of
Background and Aims: Long non-coding RNA small nucle-olar RNA host genes (SNHGs) play a critical role in the occur-rence and development of tumors. In this study, we aimed to investigate the role of SNHG4 in hepatocellular carcinoma (HCC) and its underlin