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目的调查浙江省义乌市鼠疫疫源地啮齿动物中致病性耶尔森菌的分布情况,并分析菌株的生物学特征。方法采集鼠粪便进行耶尔森菌分离,对分离获得的菌株进行生物分型、血清分型和毒力基因鉴定。结果 2005—2013年共检测3623份标本,分离到小肠结肠炎耶尔森菌74株,假结核耶尔森菌11株;耶尔森菌的检出率以春季最高(5.72%)。68株小肠结肠炎耶尔森菌有生物1A型(55株)、生物3型(1株)2个型别;血清分型O:5血清型有8株、O:8血清型有2株;毒力基因检测有29株菌检出ystB基因。结论义乌市鼠疫疫源地内未检出鼠疫菌,但分离出假结核菌和小肠结肠炎菌;在鼠疫监测同时也对其他两种致病性耶尔森菌开展监测和相关研究,对鼠疫的监测有指导意义。
Objective To investigate the distribution of pathogenic Yersinia in rodents of plague origin in Yiwu, Zhejiang Province and to analyze the biological characteristics of the strains. Methods Rat feces was collected for Yersinia separation, and the isolates were identified by typing, serotyping and virulence genes. Results A total of 3623 specimens were detected from 2005 to 2013, of which 74 strains were Yersinia enterocolitica and 11 strains were Yersinia pseudotuberculosis. The detection rate of Yersinia was the highest in spring (5.72%). Among 68 strains of Yersinia enterocolitica, there are 2 types of biological type 1A (55 strains) and biological type 3 (1 type), 8 serotypes of O: 5 serotypes, 2 strains of O: 8 serotypes ; 29 strains of virulence genes were detected ystB gene. Conclusions Yersinia pestis was not detected in the plague foci of Yiwu, but fake TB and enterocolitis were isolated. In the meantime, two other pathogenic Yersinia strains were also monitored and related studies were conducted. Monitoring is instructive.