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目的探讨鼻咽细胞复制不同时期ＤＮＡ含量与癌细胞发生发展的关系，为建立现代化诊断方法提供资料。方法本文应用流式细胞仪（ＦＣＭ）对６４例经病理诊断为鼻咽癌（ＮＰＣ）患者的鼻咽细胞进行ＤＮＡ含量测定，并与４１例慢性咽炎患者的鼻咽细胞ＤＮＡ含量进行对照分析。结果ＮＰＣ患者中非整倍体的阳性率为８４．４％；假阴性１５．６％；假阳性７．３％。ＤＮＡ合成期（ＳＰＦ）大于３０％的阳性率为８７．５％；假阴性１２．５％；假阳性９．８％。结论应用ＦＣＭ检测肿瘤细胞周期ＤＮＡ含量，具有快速、准确、自动化程度高的优点，有较好的临床应用前景。
Objective To investigate the relationship between the DNA content in nasopharyngeal cells and the development and progression of cancer cells in different periods and to provide information for establishing a modern diagnostic method. Methods The DNA content of nasopharyngeal cells in 64 patients with nasopharyngeal carcinoma (NPC) diagnosed by pathology was determined by flow cytometry (FCM). The DNA content of nasopharyngeal cells in 41 patients with chronic pharyngitis was analyzed. Results The positive rate of aneuploidy in NPC patients was 84.4%, false negative 15.6% and false positive 7.3%. The positive rate of DNA synthesis period (SPF) greater than 30% was 87.5%, false negative 12.5% and false positive 9.8%. Conclusion The application of FCM in the detection of tumor cell cycle DNA content has the advantages of rapid, accurate and high degree of automation, and has a good clinical application prospect.