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目的对滑桃树内生真菌Fusariumsp.2TnP1-2的抗菌活性次生代谢产物进行研究。方法采用硅胶柱色谱、Sephadex LH-20、制备性薄层色谱等对次生代谢产物进行分离纯化。根据理化性质和波谱分析进行结构鉴定。利用纸片扩散法对这些化合物的抗真菌及抗细菌活性进行测试。结果从该菌株PDA培养基发酵产物中分离得到3个化合物,分别鉴定为:trichosetin(-Ndemethyl equisetin,1),lateritin(4-methyl-6-(1-methylethyl)-3-phenylmethyl-1,4-perhydrooxazine-2,5-dione,2),5α,6-αepoxy-24(R)-methylcholesta-7,22-dien-3-βol(3)。结论3个化合物均为首次从该真菌的代谢物中分离得到,trichosetin和lateritin具有抗金黄色葡萄球菌活性。
Objective To study the secondary metabolites of the antibacterial activity of Fusarium sp.2TnP1-2, an endophytic fungus in the peach tree. Methods The secondary metabolites were isolated and purified by silica gel column chromatography, Sephadex LH-20 and preparative thin layer chromatography. According to physicochemical properties and spectral analysis for structural identification. The antibacterial and antibacterial activities of these compounds were tested using disk diffusion methods. Results Three compounds were isolated from the fermentation product of PDA medium of this strain and identified as trichosetin(-Ndemethyl equisetin,1) and lateritin(4-methyl-6-(1-methylethyl)-3-phenylmethyl-1,4 respectively. -perhydrooxazine-2,5-dione,2),5α,6-αepoxy-24(R)-methylcholesta-7,22-dien-3-βol(3). Conclusion The three compounds were isolated from the metabolites of the fungus for the first time. Trichosetin and lateritin have anti-Staphylococcus aureus activity.