论文部分内容阅读
目的探讨红细胞离体后,储存期内红细胞膜表面免疫分子CD58、CD59的变化。方法选取30份健康献血者的CPDA保存液保存的红细胞悬液,充分混匀,分6个组,每组分别装于一次性塑料小血袋中,每袋20 ml,置4℃±2℃储血专用冰箱中保存。分别于保存24 h、4 d、10 d、15 d、21 d、30 d时各取1小袋红细胞悬液,充分混匀,分别对红细胞表面免疫分子CD58、CD59的平均荧光强度进行检测。结果随着储存时间的延长,红细胞表面免疫分子CD58、CD59平均荧光强度逐渐减弱。结论在输血治疗中,除了纠正贫血、提高红细胞携氧能力外,还应考虑到对机体免疫功能的影响。
Objective To investigate the changes of CD58 and CD59 on the surface of erythrocyte membrane during the storage of erythrocyte. Methods Thirty healthy red blood cells were collected from CPDA preservation solution of healthy blood donors, which were divided into six groups. Each group was placed in disposable plastic small blood bag with 20 ml per bag and set at 4 ℃ ± 2 ℃ Blood storage dedicated refrigerator. One bag of erythrocytes was collected for 24 h, 4 d, 10 d, 15 d, 21 d, and 30 d respectively. The average fluorescence intensity of CD58 and CD59 on erythrocyte surface were measured respectively. Results With the extension of storage time, the average fluorescence intensity of CD58 and CD59 on the surface of erythrocytes decreased gradually. Conclusion In the treatment of transfusion, in addition to correct anemia, improve the ability of oxygen-carrying red blood cells, but also should take into account the impact on immune function.