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目的探讨血管紧张素转换酶(ACE)基因多态性和酗酒与缺血性脑卒中(IS)发病风险的关系。方法应用聚合酶链反应(PCR)和变性高效液相色谱(DHPLC)技术筛查了454例IS患者(病例组)和334例非IS患者(对照组)的ACE基因的多态分布,采用非条件Logistic回归模型分析基因型、酗酒情况与缺血性脑卒中患病的关系。结果与对照组相比,酗酒群体的DD基因型和D等位基因的频率显著性升高(P<0.05),患缺血性脑卒中的相对危险度分别为8.130和2.488;而携带有Ⅱ基因型的酗酒群体患缺血性脑卒中的相对危险度则为0.389。相反,非酗酒IS患者的ACE基因的各基因型和等位基因频率的分布与对照组相比,均无显著性差异(P>0.10)。结论携带有D等位基因的酗酒群体容易患缺血性脑卒中,但携带有Ⅰ等位基因的酗酒群体不容易患缺血性脑卒中,ACE基因与酗酒在缺血性脑卒中的发病过程中存在协同作用。
Objective To investigate the relationship between angiotensin converting enzyme (ACE) gene polymorphism and the risk of alcohol abuse and ischemic stroke (IS). Methods The polymorphism of ACE gene in 454 IS patients (case group) and 334 non-IS patients (control group) was screened by polymerase chain reaction (PCR) and denaturing high performance liquid chromatography (DHPLC) Conditional Logistic Regression Model to Analyze the Relationship between Genotype, Alcoholism and Stroke in Ischemic Stroke. Results Compared with the control group, the frequency of DD genotype and D allele of alcohol abuse group was significantly increased (P <0.05), and the relative risk of ischemic stroke was 8.130 and 2.488, respectively. While those carrying Ⅱ The relative risk for ischemic stroke was 0.389 for genotypes of alcohol abusers. In contrast, there was no significant difference (P> 0.10) in the distribution of genotype and allele frequencies of ACE genes in non-alcoholic IS patients compared with the control group. Conclusion Alcohol abusers carrying the D allele are susceptible to ischemic stroke. However, the alcoholic population carrying the I allele is not susceptible to ischemic stroke. The pathogenesis of ischemic stroke is associated with ACE gene and alcoholism There is a synergistic effect.