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A colorimetric and fluorometric dual probe based on a water-soluble polythiophene derivative (PMTPBA) was designed and synthesized. It can be applied to determination of picric acid (PA) in 100%aqueous solution. The approach relies on the formation of supramolecular polythiophene assemblies in the presence of PA through electrostatic, charge transfer andπ-πstacking interactions. This probe could be utilized for the rapid and visual detection of PA both in aqueous solution and solid support with high specificity and sensitivity. The detection limit of this sensor is as low as 5.0 × 10-8 mol/L.