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目的:检测乙肝病毒表面抗原大蛋白水平的变化,探讨其对乙肝病人诊疗状态的评价价值。方法:1042例乙肝病人以ELISA法进行BHM分组,分别对“大三阳”组、“小三阳”组、HBeAg阴性组、HBV DNA检测阴性组和HBV基因变异组进行HBV-LP测定(ELISA法)及HBV DNA检测(FQ-PCR法)。结果:1042例乙肝病人检测结果显示:L、S、EN及DN组HBV-LP与HBV DNA阳性率分别为73.13%、67.96%,72.73%、42.32%和60.40%、39.88%,除L组(P>0.05)外,其余各组相比差异显著有统计学意义(P<0.01,P<0.05);两种检测方法之间有很好的正相关性(r=0.8420);对HBV DNA检测阴性的196例标本HBV-LP检测的阳性率为68.88%。结论:HBV-LP检测可作为从蛋白水平反映HBV病人体内病毒复制程度的血清学指标,对于HBeAg阴性的乙肝病人HBV-LP检测反映病毒复制优于HBV DNA检测,并与之具有很好互补作用。
Objective: To detect the change of large protein level of hepatitis B virus surface antigen, and to evaluate its value in diagnosis and treatment of hepatitis B patients. Methods: One hundred and forty-two patients with hepatitis B were divided into two groups according to the method of ELISA: BHM group, HBsAg negative group, HBV DNA negative group and HBV gene mutation group Determination (ELISA method) and HBV DNA test (FQ-PCR method). Results: The results of 1042 hepatitis B patients showed that the positive rates of HBV-LP and HBV DNA in L, S, EN and DN groups were 73.13%, 67.96%, 72.73%, 42.32% and 60.40%, 39.88% (P <0.01, P <0.05). There was a positive correlation between the two methods (r = 0.8420). The detection of HBV DNA Negative 196 cases of HBV-LP test the positive rate was 68.88%. Conclusion: HBV-LP test can be used as a serological indicator to reflect the degree of virus replication in HBV patients from the protein level. HBV-LP test in HBeAg-negative hepatitis B patients is better than HBV DNA test in detecting HBV replication and has a good complementarity .