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目的:探讨蔡氏扶正消癥汤对人体外生长胃癌细胞SGC-7901表面黏附分子的影响以及作用的计量和效应关系。方法:流式细胞仪检测细胞膜表面E-Cadherin,CD44,CD44-v6和CD54的表达。结果:蔡氏扶正消癥汤作用48h后,SGC-7901细胞膜表面的E-Cadherin表达增高,CD44、CD44-V6和CD54表达减少。随着药物剂量增加,E-Cadherin表达阳性细胞表达逐渐升高;CD44、CD44-v6、CD54表达的阳性细胞比率逐渐下降,抑制率也逐渐升高。结论:蔡氏扶正消癥汤在抑制人胃癌细胞SGC-7901体外增殖的基础上,能够增加胃癌细胞SGC-7901表面E-Cadherin的表达,减少胃癌细胞SGC-7901表面CD44,CD44-V6和CD54的表达,并在计量和效应间存在联系,说明蔡氏扶正消癥汤能阻止胃癌SGC-7901细胞对邻近正常组织的浸润及远处转移,从而发挥抗癌作用。
Objective: To investigate the effect of Cai’s Fuzheng Xiaozheng Tang on gastric cancer cell SGC-7901 surface adhesion molecule in vitro as well as the effect of measurement and effect. Methods: Flow cytometry was used to detect the expression of E-Cadherin, CD44, CD44-v6 and CD54 on the cell membrane. Results: The expression of E-Cadherin and the expressions of CD44, CD44-V6 and CD54 on the surface of SGC-7901 cells were increased after 48 hours of treatment. The expression of E-Cadherin positive cells gradually increased with the increase of the dose of the drug. The percentage of positive cells expressing CD44, CD44-v6 and CD54 gradually decreased and the inhibition rate increased gradually. Conclusion: Chuaizhengzhengxutang can inhibit the proliferation of SGC-7901 cells in vitro and increase the expression of E-cadherin on the surface of SGC-7901 cells and decrease the expression of CD44, CD44-V6 and CD54 on the surface of SGC-7901 cells Expression, and there is a relationship between measurement and effect, indicating that Cai’s Fuzheng Xiaozhi Decoction can prevent gastric cancer SGC-7901 cells from infiltration and distant metastasis of adjacent normal tissues, and thus exert anti-cancer effects.