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幽门弯曲菌(简称CP)能产生尿素酶。作者曾报道利用胃粘膜活检标本尿素酶试验可以快速诊断Cp感染。本文报道应用尿素酶试验检查一大组病例的结果。1986年6月至1987年6月,1445例进行上消化道内镜检查的病人作为研究对象。胃镜检查时,在距幽门5cm以内的胃窦部钳取粘膜组织4块,分别进行下列检查:组织切片HE染色供组织学诊断,半革兰氏染色检查Cp,并根据细菌的数量分为0~3级;粘膜直接涂片革兰氏染色,镜检Cp;应用巧克力琼脂和弯曲菌选择性琼脂在微氧条件下培养;尿素酶试验,Cp产生的尿素酶分解尿素,生成氨,使pH升高,引起指示剂酚红颜色的变化,由正常的黄棕色变为粉红色即为阳性。记录
Campylobacter pylori (CP) can produce urease. The authors have reported using gastric mucosal biopsy specimens urease test can quickly diagnose Cp infection. This article reports the application of a urease test to examine the results of a large group of patients. From June 1986 to June 1987, 1,445 patients undergoing upper gastrointestinal endoscopy were selected as study subjects. Gastroscopy, within 4cm from the anterior pylorus mucosa of the antrum pliers to take 4, respectively, for the following checks: HE staining of histological sections for histological examination, semi-Gram staining Cp, and according to the number of bacteria is divided into 0 ~ 3 grades; Mucosal direct smear Gram stain, microscopy Cp; application of chocolate agar and Campylobacter selective agar cultured in micro-oxygen conditions; urease test, urease urease decomposition urea, ammonia, the pH Rise, causing the indicator phenol red color changes from the normal yellow-brown to pink is positive. recording