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应用电化学方法研究了基因工程酶 (L 扁桃体酸脱氢酶 )的电化学性质 ,探讨了其催化机理。以聚赖氨酸为促进剂 ,L 扁桃体酸脱氢酶黄素微区在裂解石墨棱面 (EPG ,edge planepyrolyticgraphite)电极上有两对氧化还原峰 ( -0 .4 81V和 -0 .60 5Vvs.SCE ,Tris缓冲溶液pH 7.5,扫描速度 2 0mV/s) ,显示出酶的辅基黄素单核苷酸 (FMN)与电极之间的电子传递过程。在此条件下 ,L 扁桃体酸脱氢酶黄素微区不能催化L 扁桃体酸脱氢 ,但用二茂铁甲酸和细胞色素C作为媒介体 ,能促进酶催化反应有效地进行
The electrochemical properties of genetically engineered enzymes (L-tonsil acid dehydrogenase) were studied by electrochemical methods and their catalytic mechanisms were discussed. Using polylysine as a promoter, L-amygdalin flavin microarrays showed two pairs of redox peaks (-0.481V and -0.65VVs.sCE) on the edge plane pyrolyticgraphite (EPG) electrode , Tris buffer pH 7.5, scan rate 20 mV / s) showed the electron transfer between the enzyme FIT and the electrode. Under this condition, the L-amygdalin flavin domain can not catalyze L-tonsil acid dehydrogenation, but ferrocenecarboxylic acid and cytochrome-C as intermediates can promote efficient enzymatic reactions