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目的从细胞半数抑制浓度IC50和氧化作用的角度探讨不同粒径大小纳米Fe2O3粒子细胞毒性的差异。方法将8、13和37nm3个不同尺寸的纳米Fe2O3粒子以不同剂量、作用时间作用于CHL细胞。用活细胞计数法求得各尺寸粒子的IC50,并绘制细胞生长抑制曲线。硫代巴比妥酸法测定丙二醛(MDA)含量,黄嘌呤氧化酶法测定超氧化歧化酶(SOD)活性。结果3种尺寸的纳米粒子在一定浓度范围内,剂量与细胞抑制率呈现“剂量-反应”关系,超过这一范围后,量效关系消失;在量效相关的浓度范围内,三个尺寸纳米粒子的IC50分别为:IC50(8nm)=279.8585μg/mL,IC50(13nm)=254.739μg/mL,IC50(37nm)=561.237μg/mL;纳米Fe2O3粒子可引起CHL细胞的氧化应激反应。且与作用时间有关。但MDA、SOD值变化与纳米粒子尺寸、作用剂量之间无明显的量效关系。结论不同粒径的纳米Fe2O3粒子在细胞毒性上表现出一定的差异。同时,纳米Fe2O3粒子可引起CHL细胞的氧化应激反应。从时效性分析推论其细胞毒性与氧化性存在一定的关联。
OBJECTIVE To investigate the cytotoxicity of Fe2O3 nanoparticles with different particle sizes from the perspective of IC50 of half cell concentration and oxidation. Methods Three different sizes of Fe2O3 nanoparticles of 8, 13 and 37 nm were treated with CHL cells at different doses for different time. The IC50 of each size particle was calculated by the method of viable cell count, and the cell growth inhibition curve was drawn. The content of malondialdehyde (MDA) was determined by thiobarbituric acid method and the activity of superoxide dismutase (SOD) was measured by xanthine oxidase method. Results The results showed that there was a “dose-response” relationship between the dose and the cell inhibitory rate within a certain range of concentration. After this range, the dose-response relationship disappeared. In the concentration-dependent range, The IC50 of the particles were: IC50 (8nm) = 279.8585μg / mL, IC50 (13nm) = 254.739μg / mL, IC50 (37nm) = 561.237μg / mL; Fe2O3 nanoparticles can cause oxidative stress in CHL cells. And with the role of time. However, there was no significant dose-response relationship between the change of MDA and SOD and the size and dose of nanoparticles. Conclusion Fe2O3 nanoparticles with different particle sizes show some differences in cytotoxicity. At the same time, Fe2O3 particles can cause oxidative stress in CHL cells. Inferred from the timeliness analysis of its cytotoxicity and oxidation of a certain relationship.