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目的探讨表达人干扰素α-2b的重组乳杆菌应用于阴道HSV-2感染的治疗作用。方法建立阴道感染HSV-2的小鼠模型,使用重组菌菌液于感染前24h进行预防,或感染后48h进行治疗,记录病损程度并检测小鼠阴道分泌物中HSV-2滴度。结果使用重组菌预防或治疗的小鼠症状轻且病程缩短,病灶中病毒滴度下降很快。结论表达IFNα-2b的重组乳杆菌具有较强的局部抗病毒作用,可用于预防及治疗由HSV-2感染引起的生殖器疱疹。
Objective To investigate the therapeutic effect of recombinant lactobacillus expressing human interferon α-2b on vaginal HSV-2 infection. Methods A mouse model of vaginal infection with HSV-2 was established. The recombinant strain was used to prevent it 24 h before infection or 48 h after infection. The severity of HSV-2 in the vaginal secretions of mice was recorded and the titer of HSV-2 was measured. Results The mice treated with recombinant bacteria showed mild symptoms and shorter duration of symptoms, and viral titers in the lesions dropped rapidly. Conclusion Recombinant lactobacillus expressing IFNα-2b has a strong local antiviral activity and can be used to prevent and treat genital herpes caused by HSV-2 infection.