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目的建立稳定可靠的辅助性肝移植动物模型。方法杂交犬14条8-25 kg随机分两组。供体组肝脏均采用尸体肝左叶;受体组行标准左叶切除,供肝左叶原位移植于受体体内。结果供肝热缺血时间为零,冷缺血时间平均36．3 min;灌注液的量平均2．96 L,切取修剪肝左叶时间为23-40 min。受体手术平均时间5．3 h,平均出血量140 ml,肝脏血管重建后红润柔软,5-11 min内即见胆汁从胆管中溢出。受体组术后全部存活,围手术期未用任何血管活性药物、抗生素及免疫抑制剂,术后存活超过6 h者5例,最长存活者达5．7 d。结论犬是建立辅助性肝移植模型的理想动物。
Objective To establish a stable and reliable animal model of liver transplantation. Methods 14 dogs of 8-25 kg were randomly divided into two groups. The liver of the donor group was allotted to the left lobe of the liver of the dead body. The left lobe of the donor liver was implanted in the recipient body in situ. Results The time of warm ischemia was zero, the time of cold ischemia was 36.3 minutes on average, the volume of perfusion was 2.96 L on average, and the left lobe of clipping liver was 23-40 minutes. The mean time of rectal operation was 5.3 h, with an average blood loss of 140 ml. RBC was reconstructed to be red and soft. Within 5-11 min, bile leaked from the bile duct. All patients survived after operation. Perioperative patients did not receive any vasoactive drugs, antibiotics and immunosuppressive agents. Five patients survived more than 6 hours and the longest survivors reached 5.7 days. Conclusion Dogs are the ideal animal to establish the model of liver transplantation.