论文部分内容阅读
目的探讨激活素A对小鼠腹腔巨噬细胞分泌细胞因子的影响。方法分离小鼠腹腔巨噬细胞,将其分为激活素A处理组和对照组,瑞氏-吉姆萨染色观察小鼠腹腔巨噬细胞形态学变化;流式细胞术分析小鼠腹腔巨噬细胞表面分子CD68的表达;ELISA法检测小鼠腹腔巨噬细胞分泌IL-10及TNFα的水平;Griess法检测小鼠腹腔巨噬细胞分泌NO的水平。结果经激活素A刺激后,显微镜下可见呈不规则、多边型的活化巨噬细胞增多,细胞表达巨噬细胞成熟标志CD68增加,Ⅱ型巨噬细胞(M2)产生的细胞因子IL-10及NO分泌水平升高,而Ⅰ型巨噬细胞(M1)产生的细胞因子TNFα水平无变化。结论激活素A可能主要促进小鼠Ⅱ型巨噬细胞分泌细胞因子。
Objective To investigate the effect of activin-A on the secretion of cytokines in murine peritoneal macrophages. Methods Mice peritoneal macrophages were isolated and divided into activin A treatment group and control group. Morphological changes of peritoneal macrophages were observed by Wright-Giemsa staining. Flow cytometry was used to analyze the changes of peritoneal macrophages The expression of CD68 was detected by ELISA. The levels of IL-10 and TNFα secreted by peritoneal macrophages were detected by ELISA. The levels of NO secreted by peritoneal macrophages were detected by Griess method. Results After stimulated with activin A, the number of activated macrophages increased under the microscope. CD68, a marker of maturation of macrophages, cytokines IL-10 produced by type II macrophages (M2) and NO secretion increased, while type I macrophages (M1) produced no change in cytokine TNFα levels. Conclusion Activin A may mainly promote the secretion of cytokines by type Ⅱ macrophages in mice.