论文部分内容阅读
【目的】研究分离自广东、福建、江西等15个地点的174株黑木相思(Acacia melanoxylon)根瘤菌的遗传多样性。【方法】采用16S rDNA限制性片段长度多态性分析(Restriction fragment length polymorphism,RFLP)和16S rDNA基因、持家基因(recA、atpD、glnII)系统发育分析的方进行研究。【结果】16S rDNAPCR-RFLP分析中,在70%的相似性水平上,所有供试菌株分成9个类群;16S rDNA基因和持家基因系统发育分析结果基本一致,34株代表菌株主要分布在α-变形菌纲(Alpha-Proteobacteria)的慢生根瘤菌属(Bradyrhizobium)、根瘤菌属(Rizobium)、中慢生根瘤菌属(Mesorhizobium),并与Bradyrhizobium liaoningense、Bradyrhizobium betae、Bradyrhizobium cytisi、Rizobium multihospitium、Mesorhizobium plurifarium亲缘关系较近。【结论】供试菌株被鉴定到属的水平,Bradyrhizobium、Rhizobium或Mesorhizobium为优势菌群,证明了黑木相思根瘤菌具有丰富的遗传多样性。
【Objective】 The genetic diversity of 174 Acacia melanoxylon rhizobia isolates isolated from 15 sites in Guangdong, Fujian and Jiangxi provinces was studied. 【Method】 The 16S rDNA RFLP and 16S rDNA gene and housekeeping gene (recA, atpD, glnII) phylogenetic tree were studied. 【Result】 The 16S rDNAPCR-RFLP analysis showed that all the tested strains were divided into 9 groups at 70% similarity level. The results of phylogenetic analysis of 16S rDNA gene and housekeeping gene were basically identical. The 34 representative strains mainly distributed in α- Bradyrhizobium, Rizobium and Mesorhizobium of Alpha-Proteobacteria, and are closely related to Bradyrhizobium liaoningense, Bradyrhizobium betae, Bradyrhizobium cytisi, Rizobium multihospitium, Mesorhizobium The close relationship between plurifarium. 【Conclusion】 The tested strains were identified as genus, and Bradyrhizobium, Rhizobium or Mesorhizobium were dominant populations, which proved that A. nigricans has rich genetic diversity.