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目的 探讨 IL - 4、紫杉醇对 lewis肺癌的抑制作用。方法 将 L ewis肺癌细胞 (1.2 5× 10 6 个 /鼠 )接种于 C5 7BL/ 6小鼠皮下。将 40只小鼠随机分为 、 、 、 组 ,接种后第 5天起分别给予生理盐水、IL - 4、紫杉醇、IL - 4+紫杉醇。第 18天处死动物。利用图像分析仪对肿瘤细胞核形态、DNA含量及 Ag NORs计数进行测量。结果 组核的面积为 37.0 37± 7.0 2 7μm2 ,周长为 2 2 .76 2± 4.312μm ,平均直径为 (3.136± 0 .35 1) μm,积分光密度为 (4 .2 94± 0 .992 ) ,Ag NORs计数为 (7.6 35± 1.16 0 ) ; 组核面积 (2 4.2 0 2±5 .0 12 )μm2 、周长 (18.6 2 5± 4.180 )μm、平均直径 (2 .6 42±0 .2 91) μm、积分光密度 (3.412± 0 .910 )、Ag NORs计数(5 .313± 0 .82 0 )比 组小 ,差异均有显著性 (P <0 .0 5 ) ; 组核面积 (18.373± 4.833)μm2 、周长 (16 .5 2 2± 2 .5 18)μm、平均直径 (2 .40 1± 0 .2 88) μm、积分光密度 (3.0 96± 0 .771)、Ag NORs计数 (2 .6 70± 0 .40 1)比 组小 ,差异均有高度显著性 (P<0 .0 1) ; 组核面积 (16 .6 6 4± 4.6 71) μm2 、周长(15 .90 1± 2 .70 0 ) μm、平均直径 (2 .2 83± 0 .30 8) μm、积分光密度 (2 .70 7± 0 .80 7)、Ag NORs计数 (1.5
Objective To investigate the inhibitory effect of IL - 4 and paclitaxel on lewis lung cancer. Methods L ewis lung cancer cells (1.2 × 10 6 cells / mouse) were inoculated subcutaneously in C5 7BL / 6 mice. Forty mice were randomly divided into three groups: normal saline, IL - 4, paclitaxel and IL - 4 + paclitaxel respectively on the 5th day after inoculation. The animals were sacrificed on the 18th day. Tumor cell nuclear morphology, DNA content and AgNORs counts were measured using an image analyzer. Results The area of the nucleus was 37.0 37 ± 7.0 2 7μm2, the circumference was 22.72 ± 4.312μm, the mean diameter was (3.136 ± 0.351) μm, the integral optical density was (4.22 ± 0.94) 992), and the counts of Ag NORs were (7.6 35 ± 1.16 0); the area of nucleus was (4.220 ± 5 .0 12) μm 2, the circumference was (18.6 ± 5 ± 4.180) μm, the average diameter was (P <0.05). The average optical density (0.1291) μm, integral optical density (3.412 ± 0.910) and Ag NORs (5.313 ± 0.882) Nuclear area (18.373 ± 4.833) μm 2, circumference (16.52 2 ± 2 .5 18) μm, mean diameter (2.401 ± 0.288 μm), integral optical density (3.096 ± 0.771 ) And Ag NORs (2.670 ± 0.401) were smaller than those in the control group (P <0.01). The nuclear area of the group was (16.64 ± 4.671) μm 2, (15 .90 ± 2. 70 0) μm, mean diameter (2.23 ± 0.308) μm, integral optical density (2.70 7 ± 0.80 7), Ag NORs count (1.5