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目的:制备小肠粘膜下层,用作肌腱替代物,观察其替代肌腱的过程和结果,检验体内组织工程理论的可行性。方法:取猪小肠的空肠部分,经化学处理后,将小肠粘膜下层卷曲成轴,取15只19天的罗曼鸡胚趾深屈肌腱,用酶消化方法体外分离培养肌腱细胞,并进行传代扩增。对各代肌腱细胞的活性与手术中进行比较。分别于术后第3、6、9周作为时间点,每个时间点处死8只动物切取左趾移植物,进行检测,所得数据进行处理和分析。结果:SIS替代肌腱的过程是代谢过程,经过16周,SIS为宿主肌腱完全替代,其组织结构和力学性能与正常肌腱极其接近。结论:体内组织工程理论至少在肌腱再生方面是可行的。复合细胞的SIS肌腱替代物优于单纯SIS替代肌腱的作用,SIS具有很好的生物相容性和力学强度,有可能成为肌腱组织工程的一种理想的支架材料。
OBJECTIVE: To prepare the small intestinal submucosa and use it as a tendon substitute to observe its process and result of replacing the tendon, and to test the feasibility of in vivo tissue engineering theory. Methods: The jejunum part of pig intestine was taken. After chemical treatment, the submucosa of small intestine was curled into an axis. Fifteen 19-day-old toe deep flexor tendons were obtained. The tendon cells were isolated and cultured in vitro by means of enzymatic digestion. increase. The activity of each generation of tendon cells and surgery were compared. At the 3rd, 6th and 9th week after operation as the time points, 8 animals were sacrificed at each time point for the left toe graft. The data were processed and analyzed. Results: The process of SIS replacing tendon was metabolic process. After 16 weeks, SIS was a complete replacement of the host tendon and its tissue structure and mechanical properties were very close to those of normal tendon. Conclusion: In vivo tissue engineering theory is at least feasible in tendon regeneration. The SIS tendon substitute of composite cells is superior to that of simple SIS alone. SIS has good biocompatibility and mechanical strength and may be an ideal scaffold for tendon tissue engineering.