【摘 要】
：
被子植物的AP3家族属于MADS-box基因中的B功能基因,其在花器官形成和生殖发育中有重要的作用。为研究兰花MADS-box基因的表达与花器官发育之间的关系,以文心兰(Oncidium Mill
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被子植物的AP3家族属于MADS-box基因中的B功能基因,其在花器官形成和生殖发育中有重要的作用。为研究兰花MADS-box基因的表达与花器官发育之间的关系,以文心兰(Oncidium Milliongolds)为材料,本研究通过RT-PCR方法克隆到一个花发育相关的MADS-box基因,通过该基因在花芽不同发育时期和不同组织的表达模式,推测该基因对花器官形成所起的调控作用,为兰花花器官发育的分子调控机制进一步的研究奠定基础,为兰花分子育种提供理论依据。本研究主要结果如下：(1)通过RT-PCR分离得到OAP3基因cDNA的全长,该基因全长799bp,包括一个612bp的CDS框,编码一个长度为203个氨基酸的蛋白质。在GenBank登录(登录号：GU644447)。进化树分析表明OAP3属于花器官发育B类基因,但该基因却不存在B类基因C-端共有的结构域。(2)文心兰OAP3基因蛋白属于AP3-like基因家族中的paleoAP3系列,与其它物种相比,氨基酸序列比对发现该序列与蝴蝶兰PeMADS5的氨基酸序列同源性高达78%,与单子叶植物水稻MADS16和百合LMADS1的氨基酸序列同源性均为62%,明显高于与双子叶植物拟南芥AP3(50%)和金鱼草DEF(50%)的同源性。(3)OAP3基因编码的蛋白质推测其分子量为23661.1D,理论等电点为9.49。其中,碱性氨基酸占总氨基酸的18.23%,酸性氨基酸占总氨基酸的13.80%,疏水氨基酸占总氨基酸的33.00%,极性氨基酸占总氨基酸的34.97%。(4) qRT-PCR表达分析表明：从抽花开始,随花芽的生长,该基因相对表达丰度呈明显上升趋势；OAP3在萼片、花瓣、唇瓣、雄蕊、子房以及营养器官叶片和花茎中都表达。
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