The first aim of this experiment was discussing factors influenced superovulation, perfecting the dairy cattle superovulatory system, improving the superovulatory results;the second one was discussing the production of dairy cattle sexing-embryos, establishing methods of artificial insemination for superovulation using sexing sperm. The follicular wave was induced by estrogen, progesterone (P4) and vaginal-bolt when superovulated, different kinds of vaginal-bolt, progesterone, estrogen, FSH, methods of superovulation, repeated superovulation, season effect, donor age etc. were compared in this paper to discuss factors that affected superovulation;sexing sperm was also analyzed for different inseminated site and compared the effects of the traditional sperm and sexing sperm for superovulatory results. The results displayed as follows:1. 50mg or 100mg progesterone (P4) could induce follicular wave efficiently, there were no differences in estrus synchronization and superovulatory results between the two groups (P>0.05);the progestin release device (PRD)group showed estrus 10h earlier than the sponge blot(BI) group and had better superovulatory result than PRD group markedly(P<0.05);the home-made "NiuHuan" could replace CIDR(New Zealand) and DIB(Argentina) for estrus synchronization and superovulation of cattle.2. Group estradiol benzoate (EB) 2mg could induce new follicular wave emergence successfully and attain perfect results than the other two groups (group lmg and 5mg).3. The excellent heifers superovulated repeatedly (group A) had a better superovulatory results than bad heifers (group B) markedly (P<0.05);heifers superovulated repeatedly with more than 60% useable embryo rate (group C) had a better superovulatory results than heifers with 30% useable embryo rate (group D);heifers superovulated in Jul were less than the other Jan and Oct group at the average useable embryo number (P<0.05), more than the other two groups at the average non-fertilized number.4. 13-15 month old heifers could be superovulated and produce useable embryos.there were no differences markedly with cows at the superovulatory results. So it could be applied in the embryo production commercially, quicken the genetic development and improve the gene amended speed and excellent breeder’s reproduction.5. There were no marked differences at superovulatory results when injected with twokinds of FSH (porcine-FSH and ovine-FSH, P>0.05);there were no differences between the management of degressive injection and equal injection for superovulatory results, and could both be selective methods.6. The traditional group had higher useable embryo rate than sexing sperm group (P<0.05), lower non-useable rate and non-fertilized rate than sexing sperm group (P<0.05,P<0.01);the pregnant rate and calving rate of sex-control embryos were not different significantly with traditional ones(P>0.05), higher than sex-control ones at the female rate of calving(P<0.05).7. It,was an optimal method that heifers inseminated with sexing sperm 12h and 24h after the estrus, it was higher at useable embryo rate than heifers inseminated with sexing sperm 24h and 36h(P<0.05),but lower at un-useable rate and non-fertilized rate than heifers inseminated with sexing sperm 24h and 36h(P<0.05;P=0.07).