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The methylation and the numbers of CpG in Hoxc8 exon-1 of Mongolia sheep with vertebrae 13 and 14 were investigated by immuno-precipitation real-time quantitative PCR (IRQP) and bisulfite-sequencing PCR (BSP).It indicated that the percentage of methylated CpG in vertebra 13 was much lower than that in vertebra 14 (p<0.01).