Construction and Analysis of nlp4 Mutant Strains in the Vascular Wilt Fungus Verticillium dahliae

来源 :中国植物病理学会2014年学术年会 | 被引量 : 0次 | 上传用户:abc135abc
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  Necrosis-and ethylene-inducing-like protein (NLP) is a plant pathogenic factor.Here,we identified a nep gene that encodes a type Ⅱ NLP in Verticillium dahliae, a devastating soil-borne pathogen, and named nlp4.Colony morphology, growth rate, spore production of nep knockout mutant and wild-type strains were compared and found to differ significantly.The degree of leaf disease and disease spot-size induced by knockout mutants were consistent with that induced by wild-type, indicating that nlp4 does not affect V.dahliae pathogenicity to cotton host.The results of this study provide a basis for the further study of the pathogenesis of Verticillium wilt.
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