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CRISPR/Cas9 targeting systems have been applied to a variety of species.However, most CRISPR/Cas9 systemsreported for plantscan only modify one or a few target sites.Here, we report a robust CRISPR/Cas9 vector system, utilizing a plant codon-optimized Cas9 gene,for convenient and high-efficiency multiplex genome editing in monocot and dicot plants.