The separation, detection, and quantification of various contaminants in food present significant challenges to analytical chemists.These challenges are heightened due to the diversity and complexity of molecules present in complicated food matrices, which have attracted the interest of both the academic and industrial communities.Improved sample preparation methods are required to solve these formidable analytical challenges.This presentation covers modem food safety applications using online sample extraction technology, eliminating offline sample preparation to separate target analytes from extremely complex food matrices and their impact in the area, including Veterinary drugs and chemicals in seafood, antibiotics in honey,-agonists in animal meat and pesticides analysis (Fig.1).