[Backgroud]γ-oryzanol is one of the most important bioactive substances in rice bran oil that can be used for food, pharmaceutical and cosmetic fields.To investigate the proliferative and protective effects of γ-oryzanol on L02 cells.[Methods]The model of oxidative damage was set up on L02 cells induced by H2O2.The cell viability, the contents of malondialdehyde (MDA) and glutathione (GSH), the activities of superoxide dismutase (SOD) and catalase (CAT), and the level of ROS were assayed by adding different concentration of γ-oryzanol into L02 cells in vitro.[Results]The cell viability was enhanced significantly and the levels of ROS and MDA were significantly decreased, whereas the content of GSH and the activities of CAT and SOD were significantly increased by 0.1-0.4 mmol/L of γ-oryzanol pretreatment after H2O2-induced oxidative damage in a dose dependent manner.[Conclusion]γ-oryzanol can effectively attenuate H2O2-induced oxidative damage at the dose of 0.1-0.4 mmol/L and maintain the normal physiology of the cells.