【摘 要】
：
Supercritical fluid extraction (SFE) and high-speed counter-current chromatography (HSCCC) were successfully used for extraction, separation and purification of alkaloids from corydalis decumbens.The
【机 构】
：
State Key Laboratory of New Drug and Pharmaceutical Process Shanghai Institute of Pharmaceutical Ind
【出 处】
：
2012上海中医药与天然药物国际大会
论文部分内容阅读
Supercritical fluid extraction (SFE) and high-speed counter-current chromatography (HSCCC) were successfully used for extraction, separation and purification of alkaloids from corydalis decumbens.The preparative SFE system under the conditions of 35MPa, 70℃, a sample particle sized 40-80 mesh and modified CO2 with dehydrated alcohol.HSCCC separation was used under the conditions of the optimal two-phase solvent system, which was composed of a mixture of chloroform- methanol-0.2M HCL (4:1:2, v/v) by eluting the lower mobile phase at a flow-rate of 2 ml/min and a revolution of 800 rpm.Three alkaloids were isolated by HSCCC and analyzed by high performance liquid chromatography (HPLC).Those alkaloids yielded total of 87.10 mg of xtw-2 (d-tetrahydropalmatine) at 99.7％ purity, 52.25 mg of xtw-6 ((+)-Kikemanine) at 99.0% purity and 115.40 mg of xtw-4 (Protopine) at 99.48% purity.Those chemical structures were identified by UV, IR, MS, NMR.It should be mentioned that the K-value of HSCCC for xtw-4 (Protopine) is not 0.5 to 2 as normal but 10.For its large K-value, its retention time is long.While other undesired compounds were eluted out by mobile phase, the purified Protopine was obtained from stationary phase.Present study provides a new method of separation with HSCCC.
其他文献
目的：建立鸡蛋参鉴别方法。 方法：用薄层色谱法鉴别鸡蛋参。固定相：硅胶G；展开剂：乙酸乙酯。甲醇-水(10：2：1)；显色剂：10%硫酸乙醇溶液；检出：在105℃加热数分钟，置紫外光灯(365nm)下检视。 结果：鸡蛋参供试品色谱中，在与对照药材色谱相应的位置上，显相同颜色的斑点。 结论：本法可用于藏药鸡蛋参的薄层鉴别。
Aim: The potential of red clover extract in the prevention of lipid disorder has attracted increasing attention increasingly in recent years.In this study, we determined whether and how red clover ext
Objective Osteoporosis (OP) is a kind of bone diseases that affects individuals worldwide and is particularly prevalent among elderly postmenopausal women.Many drugs are currently available for treati
目的：用反相高效液相色谱法对10种沙棘的叶中槲皮素、山柰素和异鼠李素的含量进行比较测量。 方法：采用Shim-pack VP-ODS色谱柱(4.6ram×150mm,5μm)；流动相为甲醇-0.4%磷酸水溶液(50：50),流速1.0ml/min；检测波长370nm；柱温30℃.进样量10μl。 结果：槲皮素、山柰素和异鼠李素分别在1.67～106.6μg/ml,0.51～32.64μg/ml
Objective: Determination of P.hookeri in different medicinal parts of the total saponins content and compare the differences in different parts.Method: With Ursolic acid as a reference substance, the
本文以道地药材黄连为研究对象，对其生长环境进行分析，运用ISSR等方法考察其遗传型，运用HPLC、1H-NMR及NIR等方法对化学型进行分析，针对清热燥湿，止消渴功效，从抗内毒素、抗微生物的生物热力学，以及治疗糖尿病相关的胰岛素抵抗生物效应等角度研究其药效型，并综合分析其相关性，初步建立了基于黄连生态型、基因型、化学型和药效型的道地药材品质评价方法，同时建立了结合道地性的黄连规范化质量标准，为构建
目的：优选益脑康颗粒的提取工艺(以提取的浸膏量及浸膏中黄芪甲苷的含量为定量指标，研究益脑康颗粒的最佳提取方法)。 方法：采用正交试验法进行优选方法，以浸膏量和高效液相色谱法测定浸膏中黄芪甲苷的含量为评价指标，进行正交设计试验。 结果：优选的水提工艺为提取3次，每次加15倍量水，每次提取0.5h。 结论：该方法科学合理，结果稳定可靠，可用于该制剂的提取工艺研究。
中药及中药复方是一个复杂系统，中药药效物质是中医药发挥临床治疗作用的基础，中药药效筛选和评价方面的新技术新方法引进已成为学术界关注的问题。斑马鱼作为新型模式动物的优势正在逐渐被人们所认识，应用的领域也越来越宽广，其在中药药效筛选和评价中的应用日见广泛，并逐步扩展至毒性评价、药物代谢等多个方面。本文介绍了近年国内外斑马鱼模型在中医药研究中的应用概况，以期有利于中医药基础研究融入国际学术舞台。
By the year of 1998, there were already 451 kinds of medicinal fungi explored from traditional medicines in China.In the New Chinese Patent Medicines published in 2002, there were 539 prescriptions co