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Aim: To optimize the influential factors on simulation calcium oxalate calculus model in vitro with the targets of the CaOxa crystal growth rate and the ratio of CaOxa crystals.Methods: To establish the model of calcium oxalate stone agar gel system by evaluating the influential factors which include the concentration of agar gel, calcium chloride, sodium oxalate, the incubation time and temperature in orthogonal test and each factor with three levels, affecting formation of calcium oxalate stone under the microscope.