As a traditional indicator for HCC(hepatocellular carcinoma)testing,diagnostic value of AFP(alpha fetoprotein,AFP) has been evaluated and questioned.Previous reports suggest that AFP heterogeneity with various glycans can be used as an independent biomarker.Currently,the major methods to detect AFP glycol-isoforms are based on specific affinity of different lectins to various oligosaccharide chains.These detection methods have their limitations.Here,we propose a strategy combining immunoaffinity enrichment with multiple reaction monitoring(MRM) technology to quantitatively analyze various oligosaccharides and the intact glycopeptides of serum AFP.