【摘 要】
：
卵黄原蛋白是胚胎发育的营养物质之一.蜕皮激素是调节家蚕生理、发育以及行为等的重要激素之一.在前期研究中,我们已经阐明了BmBrC-Z2可以结合在BmVg基因启动子上应答蜕皮激素调节BmVg的转录表达.本研究信息学分析发现位于-186～-211nt的BrC-Z2 CRE邻近存在POU元件.通过细胞转染、EMSA和Chip实验分析显示,BmPOUM2元件应答蜕皮激素调控BmVg启动子的活性有影响,而且
【机 构】
：
家蚕基因组生物学国家重点实验室,西南大学,重庆,400716 重庆新型蚕丝材料工程与技术研究中心,
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卵黄原蛋白是胚胎发育的营养物质之一.蜕皮激素是调节家蚕生理、发育以及行为等的重要激素之一.在前期研究中,我们已经阐明了BmBrC-Z2可以结合在BmVg基因启动子上应答蜕皮激素调节BmVg的转录表达.本研究信息学分析发现位于-186～-211nt的BrC-Z2 CRE邻近存在POU元件.通过细胞转染、EMSA和Chip实验分析显示,BmPOUM2元件应答蜕皮激素调控BmVg启动子的活性有影响,而且BmPOUM2转录因子可以特异结合该元件.qPCR和免疫组化定位显示,BmPOUM2基因在雌蚕变态期前后的转录趋势与BmVg基因一致；BmPOUM2蛋白的信号主要集中在细胞核中,而BmVg蛋白信号则在细胞质中,这也暗示了BmPOUM2作为转录因子可调控BmVg转录.在BmE-SWU1细胞系中同时过表达BmPOUM2与BmBrC-Z2时,BmVg启动子响应蜕皮激素的活性会更高；GST-pull down及免疫共沉淀实验显示,BmPOUM2与BmBrC-Z2可以结合.RNA干涉下调家蚕蛹期BmPOUM2时,会影响BmVg的表达下调,而且卵的发育异常.总之,结果表明BmPOUM2与转录因子BmBrC-Z2协调应答蜕皮激素调控BmVg转录.
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