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5. 5-Aza诱导脐带间充质干细胞向成肌细胞分化

5-Aza诱导脐带间充质干细胞向成肌细胞分化

来源 :2014中国妇产科学术会议 | 被引量 : 0次 | 上传用户：nmgbmm

【摘 要】

：

目的 体外证实人脐带间充质干细胞(human umbilical cordmesenchymal stem cells,hUCMSCs)可向成肌细胞分化,为hUCMSCs治疗压力性尿失禁奠定理论基础.方法 通过贴壁法分离脐带间充质干细胞;流式细胞仪鉴定hUCMSCs;透射电镜及原子力电镜观察细胞超微结构;CCK-8试剂盒筛查不同浓度的5-氮杂胞苷(5-Aza)对hUCMSCs增殖的影响;5-Aza

【作 者】

：

蓝建发 罗新 宋泓 石海燕 

【机 构】

：

暨南大学附属第一医院妇产科,广州510630 兰州大学第二医院妇产科,兰州

【出 处】

：

2014中国妇产科学术会议

【发表日期】

：

2014年8期

【关键词】

：

间质干细胞 结蛋白 重链肌球蛋白 CCK-8 5-氮杂胞苷 

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论文部分内容阅读

目的 体外证实人脐带间充质干细胞(human umbilical cordmesenchymal stem cells,hUCMSCs)可向成肌细胞分化,为hUCMSCs治疗压力性尿失禁奠定理论基础.方法 通过贴壁法分离脐带间充质干细胞;流式细胞仪鉴定hUCMSCs;透射电镜及原子力电镜观察细胞超微结构;CCK-8试剂盒筛查不同浓度的5-氮杂胞苷(5-Aza)对hUCMSCs增殖的影响;5-Aza诱导hUCMSCs向成肌细胞分化;RT-PCR检测成肌细胞特异基因结蛋白(desmin)和重链肌球蛋白(myosin heavy chain) mRNA表达;westernblot检测desmin和myosin heavy chain蛋白表达.

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